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Biology Question 115 – NEET-UG 2023

In tissue culture experiments, leaf mesophyll cells are put in a culture medium to form callus. This phenomenon may be called as -

Plant cells exhibit totipotency, meaning a single plant cell can develop into a whole plant under suitable conditions.

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Ninja StrategyProcess Definition Recall

Quickly recall the definitions of differentiation, dedifferentiation, and redifferentiation in the context of plant tissue culture to identify the correct term for a specialized cell reverting to an unspecialized state.

Step 1: Analyze the Process Described✦ Active

The question describes leaf mesophyll cells, which are differentiated (specialized) plant cells, being placed in a culture medium. In this medium, they form a callus, which is an undifferentiated mass of cells.

Step 2: Define Key Biological Terms○ Expand

1. **Differentiation:** The process by which a less specialized cell becomes a more specialized cell type. 2. **Dedifferentiation:** The process by which a differentiated cell reverts to a less differentiated or undifferentiated state, often becoming meristematic again. 3. **Redifferentiation:** The process by which a dedifferentiated cell again differentiates into a specialized cell type. 4. **Development:** A broad term encompassing growth, differentiation, and maturation. 5. **Senescence:** The process of aging in cells, tissues, or organisms.

💡 Teacher's Secret Hint

Remember the sequence: Differentiation -> Dedifferentiation -> Redifferentiation in plant tissue culture.

Step 3: Identify the Correct Phenomenon○ Expand

Since differentiated leaf mesophyll cells are reverting to an undifferentiated callus state, the phenomenon is called dedifferentiation.

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