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Biology Question 150 – NEET-UG 2023

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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below :
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Ninja Strategy•Process Endpoint Analysis

The final step in this sequence must be the introduction of the recombinant DNA into a host cell (A). This immediately eliminates options (2) and (4), which do not have A as the last step, narrowing the choice to (1) and (3).